Composite

Part:BBa_K2622046:Experience

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


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Applications of BBa_K2622046

Amplified gene sequence of SurA was ligated into a plasmid pET28b. SurA was overexpressed in cytoplasm by inducing with 0,1mM IPTG and growing E. coli cells in 16*C overnight. Cells pelleted, lysed by sonication and purified with Ni-NTA column. This yielded a highly pure protein. SurA was used in a folding assay, which showed that BamB and BamCDE lipoproteins can assemble BamA into the membrane with an efficiency higher than 50% in time range of only two hours.

T--Vilnius-Lithuania--Fig11_BAM_compl.png

User Reviews

UNIQ718ebd92dbab9f71-partinfo-00000000-QINU UNIQ718ebd92dbab9f71-partinfo-00000001-QINU